Research Article

Modulatory Effect of Fraction of Dryopteris dilatata on Alloxaninduced Oxidative Stress, Hepato-nephro Injury in Wistar Rat Models

Abstract

The present study investigates the protective and modulatory effects of a bioactive fraction of Dryopteris dilatata against oxidative stress and hepato-renal damage induced by alloxan in Wistar rat models. Alloxan, a known diabetogenic agent, was administered to induce oxidative stress and subsequent hepatic and renal injury, simulating diabetic complications. Experimental rats were divided into control, alloxan-induced, and treatment groups receiving varying doses of the plant extract fraction. Biochemical parameters, including markers of oxidative stress (MDA, SOD, CAT, GPx), liver function (ALT, AST, ALP, bilirubin), and renal function (urea, creatinine, uric acid), were assessed. Histopathological evaluations of liver and kidney tissues were also conducted. The results demonstrated that treatment with the Dryopteris dilatata fraction significantly ameliorated oxidative stress, restored antioxidant enzyme activities, and improved liver and kidney function markers compared to the alloxan-only group. Histological observations further confirmed the protective effect, showing reduced cellular degeneration and improved tissue architecture. These findings suggest that the Dryopteris dilatata fraction exhibits potent antioxidant and organ-protective properties, making it a potential therapeutic candidate for managing oxidative stress-related hepatic and renal complications.