Research Article
Lignolytic Isolates from Mixed Waste Digester and a Metagenomic Analysis of the Microbial Community
- By Magdaline Joseph Kwaji, Sarah Nuhu Kase, Dennis Amaechi, Christy Chinyere Fredrick, Fatima Dantanko, Onyeka Chioma Favour, Garba Ninani, Jubilee Dauda, Agnes Aliyu, Jamilah Ibrahim Suleiman, Jonah Danladi - 21 May 2026
- Journal of Public Health and Environmental Research, Volume: 2(2026), Issue: 1(January-June), Pages: 31 - 35
- https://doi.org/10.58612/jpher216
- Received: 18.02.2026; Accepted: 15.05.2026; Published: 21.05.2026
Abstract
This research showed the requisite necessity of lignolytic microorganisms derived from waste feed-stock in the environment, industry, (medicine, pharmacy, textile) and metabolic process confirming their degradability and gas producing ability. This study aimed at the isolation and identification of lignolytic microrganisms from mixed waste using molecular characterization and a metagenomic approach on waste derived microbiome and their potentials in gas production and waste conversion. The organisms of interest are the ligninolytic and gas producing microbes (methanogens). Metagenomics of the cow dung substrate was also done. Samples (Fruit and vegetable residuez+wood waste+animal waste) were collected from AMAC Relocation market, Lugbe, F.C.T. and analysed at the International Institute for Tropical Agriculture (IITA) Oyo state. Mineral solution containing CuSO4 was used to identify cellulose degraders. Their degradative ability was confirmed by the formation of a clear zone. Twenty-four anaeroboic bacteria were isolated from mixed waste using molecular characterization out of which six bacteria species were identified as Methanothermobacter hermautotrophicu CCSD, Methanobacteriaceae archaeon KR-H07 A06, Methanobacteriaceae archaeo RK-H07, and Candidatus methanoperedenaceae GB50, Methanothermobacter thermautotrophicus NPK, Candidatus methanoperedenaceae GB37. Biogas yield increased for most of the days when compared from (3.0 to 8.0 mL), (5.0 - 24.0 mL) on day 30. All the methanogens identified produce gas. A total of 180 bacterial species were found in the substrate in which Bacteroidota (26.91%), Fermicutes (25.87%) and Proteobacteria (7.24%) predominated. Seventy (70.0%) were anaerobes while 30% were aerobes. Clostridium were the highest having (28.8%) amongst the anaerobic bacterial community while Acidobacteriota was the least (0.01%). Eschericia coli was the highest aerobic species having (14.5%) while Streptomyces was the least (0.01%).